antibody rabbit anti-p-mtor (ser2448)  (Cell Signaling Technology Inc)

Journal: Molecular Medicine Reports

Article Title: Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway

doi: 10.3892/mmr.2025.13545

Figure Lengend Snippet: Antcin K suppresses PI3K, Akt and mTOR signaling. Western blotting analysis of levels of p-p85, Akt and mTOR after 24 h incubation with antcin K. *P<0.05 vs. control. p-, phosphorylated.

Article Snippet: The phosphorylated (p)-p85 (1:2,000; cat. no. 4228S), p-Akt (1:2,000; cat. no. 4060S), p-mTOR (1:2,000; cat. no. 5536S), mTOR (1:2,000; cat. no. 2983S) and p-p65 (1:2,000; cat. no. 3033) antibodies were obtained from Cell Signaling Technology, Inc. Antibodies for MMP-7 (1:500; cat. no. sc-515703), Akt (1:500; cat. no. sc-5298) and p85 (1:500; cat. no. sc-1637) were purchased from Santa Cruz Biotechnology, Inc. p65 (1:2,000; cat. no. GTX102090) and β-actin (1:5,000; cat. no. GT5512) antibodies were purchased from GeneTex International Corporation.

Techniques: Western Blot, Incubation, Control

Journal: Molecular Medicine Reports

Article Title: Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway

doi: 10.3892/mmr.2025.13545

Figure Lengend Snippet: Inhibitory effect of antcin K on chondrosarcoma cell motility. Antcin K inhibits chondrosarcoma cell migration and invasion by decreasing MMP-7 expression by downregulating PI3K, Akt, mTOR and NF-κB signaling cascades. Image created using BioRender.com. p-, phosphorylated.

Article Snippet: The phosphorylated (p)-p85 (1:2,000; cat. no. 4228S), p-Akt (1:2,000; cat. no. 4060S), p-mTOR (1:2,000; cat. no. 5536S), mTOR (1:2,000; cat. no. 2983S) and p-p65 (1:2,000; cat. no. 3033) antibodies were obtained from Cell Signaling Technology, Inc. Antibodies for MMP-7 (1:500; cat. no. sc-515703), Akt (1:500; cat. no. sc-5298) and p85 (1:500; cat. no. sc-1637) were purchased from Santa Cruz Biotechnology, Inc. p65 (1:2,000; cat. no. GTX102090) and β-actin (1:5,000; cat. no. GT5512) antibodies were purchased from GeneTex International Corporation.

Techniques: Migration, Expressing

Journal: iScience

Article Title: STEAP3 promotes triple-negative breast cancer growth through the FGFR1-mediated activation of PI3K/AKT/mTOR signaling

doi: 10.1016/j.isci.2025.112526

Figure Lengend Snippet: Altering STEAP3 expression in TNBC cells modulates PI3K/AKT/mTOR pathway activity (A–D) Western immunoblotting was used following the KD of STEAP3 to assess the relative levels of p-PI3K/PI3K, p -AKT/AKT, and p -mTOR/mTOR in MDA-MB-231 cells. (E–H) A similar approach was used to analyze MDA-MB-468 cells following STEAP3 KD. (I–L) The effects of STEAP3 OE on the levels of components of the PI3K/AKT/mTOR signaling pathway in BT-549 cells. Protein levels are given as ratios normalized to the total levels of the corresponding protein. Data are presented as mean ± SD (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: p -mTOR (Ser2448) , Cell Signaling Technology , Cat# 5536T; RRID: AB_10691552.

Techniques: Expressing, Activity Assay, Western Blot

Journal: iScience

Article Title: STEAP3 promotes triple-negative breast cancer growth through the FGFR1-mediated activation of PI3K/AKT/mTOR signaling

doi: 10.1016/j.isci.2025.112526

Figure Lengend Snippet: STEAP3 inhibits in vivo TNBC tumor growth (A–D) Significant reductions in tumor weights and volumes were evident in the shSTEAP3 group as compared to shCtrl. (E and F) STEAP3 levels in tumor xenografts were confirmed through Western immunoblotting. (G–J) Western immunoblotting was used to assess FGFR1 and STEAP3 protein levels and those of p-PI3K/PI3K, p -AKT/AKT, and p -mTOR/mTOR within tumor xenografts (“+” indicates high expression; “-” indicates the low expression of STEAP3 and FGFR1). (K–L) Representative IHC staining images showing STEAP3 expression in MDA-MB-231 xenograft tumors from shCtrl and shSTEAP3 groups, with quantification of IHC scores for 10 paired xenograft tumors presented as a paired dot plot. Scale bars: 25 μm. (M) The knockdown of STEAP3 in MDA-MB-231 led to a reduction in PCNA and Ki67 protein levels relative to shCtrl cells in xenograft tumor. Scale bars: 50 μm. (N) The proposed functions of STEAP3 in TNBC. Data are presented as mean ± SD (∗ p < 0.05, ∗∗ p < 0.01. ∗∗∗ p < 0.001).

Article Snippet: p -mTOR (Ser2448) , Cell Signaling Technology , Cat# 5536T; RRID: AB_10691552.

Techniques: In Vivo, Western Blot, Expressing, Immunohistochemistry, Knockdown